The iris is a thin circular diaphragm, which lies between the cornea and the lens of the human eye. A front-on view of the iris is shown below. The iris is perforated close to its centre by a circular aperture known as the pupil. The function of the iris is to control the amount of light entering through the pupil, and this is done by the sphincter and the dilator muscles, which adjust the size of the pupil. The average diameter of the iris is 12 mm, and the pupil size can vary from 10% to 80% of the iris diameter.

The iris consists of a number of layers; the lowest is the epithelium layer, which contains dense pigmentation cells. The stromal layer lies above the epithelium layer, and contains blood vessels, pigment cells and the two iris muscles. The density of stromal pigmentation determines the colour of the iris. The externally visible surface of the multi-layered iris contains two zones, which often differ in colour. An outer ciliary’s zone and an inner pupillary zone, and these two zones are divided by the collarette – which appears as a zigzag pattern.

Formation of the iris begins during the third month of embryonic life. The unique pattern on the surface of the iris is formed during the first year of life, and pigmentation of the stroma takes place for the first few years. Formation of the unique patterns of the iris is random and not related to any genetic factors. The only characteristic that is dependent on genetics is the pigmentation of the iris, which determines its colour. Due to the epigenetic nature of iris patterns, the two eyes of an individual contain completely independent iris patterns, and identical twins possess uncorrelated iris patterns.